Design Signatures

The Design Signatures web tool assists in the design of primers for sequencing, HRM, or FLP analysis with the goal of obtaining diverse amplicon signatures.

F.A.Q.        Inputs        Outputs

Experimental application: [?]
Sequencing
High Resolution Melting (HRM) analysis
Fragment Length Polymorphism (FLP) analysis

Primers: [?] Length - nucleotides with up to permutation(s)

PCR Product Amplicon: [?] Length - base pairs

Reaction Conditions: [?]
[Na⁺] = mM,  [Mg⁺⁺] = mM,  [dNTPs] = mM
Annealing Temperature = °C,  [Primers] = nM

Restriction enzymes to consider for HRM or FLP analysis (optional): [?]
AatII Acc65I AccI AciI AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AluI AlwI AlwNI ApaI ApaLI ApeKI ApoI AscI AseI AsiSI AvaI AvaII AvrII BaeGI BamHI BanI BanII BbsI BbvCI BbvI BccI BceAI BciVI BclI BcoDI BfaI BfuAI BfuCI BglI BglII BlpI BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BslI BsmBI BsmFI BsmI Bsp1286I BspCNI BspDI BspEI BspHI BspQI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssKI BssSI BstAPI BstBI BstEII BstNI BstUI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsCI BtsI BtsIMutI Cac8I CviAII CviKI-1 CviQI DdeI DraI DraIII DrdI EaeI EagI EarI EciI Eco53kI EcoNI EcoO109I EcoP15I EcoRI EcoRV FatI FauI Fnu4HI FokI FseI FspI HaeII HaeIII HgaI HhaI HincII HindIII HinfI HinP1I HpaI HphI Hpy166II Hpy188I Hpy188III Hpy99I HpyAV HpyCH4III HpyCH4IV HpyCH4V KasI KpnI MboII MfeI MluCI MluI MlyI MmeI MnlI MscI MseI MslI MspA1I MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIII NlaIV NmeAIII NotI NruI NsiI NspI Nt.BstNBI PacI PaeR7I PciI PflFI PflMI PluTI PmeI PmlI PpuMI PshAI PsiI PspGI PspOMI PspXI PstI PvuI PvuII RsaI RsrII SacI SacII SalI Sau96I SbfI ScaI ScrFI SexAI SfaNI SfcI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqaI TfiI Tsp45I TspMI TspRI XbaI XcmI XmnI ZraI

Choose FASTA file (limit 50 MB): [?]