Design FISH Probes

Use DECIPHER's Design Probes web tool to design the optimal FISH probes for targeting one group of RNA sequences in the presence of many non-target groups. If you are looking for genus-specific 16S probes then also try our 16S Oligos tool.

F.A.Q.        Inputs        Outputs

Probes: [?] Length - nucleotides with up to permutation(s)

Hybridization Conditions: [?]
[Na⁺] = M,  [Formamide] = % v/v,  [Probes] = nM
Equilibrium Temperature = °C, Hybridization Efficiency ≥ 90%80%70%60%50%40%

Target group: [?] with at least % coverage

Target molecule: [?]
SSU rRNA LSU rRNA Other RNA

Search comprehensive sequence database for potential non-targets: [?]
No Yes Yes, and exclude potential target genera

Choose aligned FASTA file (limit 50 MB): [?]