Find Chimeras

The FindChimeras function is described in: For an in-depth tutorial on finding chimeras, see the "Finding Chimeric Sequences" vignette, available from the Documentation page.

Note

Chimeras are sequence artifacts due to incomplete template elongation during PCR. We now recommend using a high-fidelity polymerase, such as KAPA HiFi, instead of removing chimeras after the fact. High processivity polymerases largely avoid chimera formation. Alternatively, it is possible to use greatly extended (≥ 3 minute) elongation times with Taq polymerase, which has relatively low processivity. In our opinion, these experimental solutions are preferable to post hoc bioinformatic chimera removal.

Please refer to this publication more information (see Fig. 4):